Suggestions by @arachnick28

Rhino

as Spider-Man in What Fictional Character has the best rogues gallery?

Mysterio (Quentin Beck)

as Spider-Man in What Fictional Character has the best rogues gallery?

Vulture (Adrian Toomes)

as Spider-Man in What Fictional Character has the best rogues gallery?

Carnage (Cletus Kasady)

as Spider-Man in What Fictional Character has the best rogues gallery?

Venom

as Spider-Man in What Fictional Character has the best rogues gallery?

Doctor Octopus (Dr. Otto Octavius)

as Spider-Man in What Fictional Character has the best rogues gallery?

Green Goblin (Norman Osborn)

as Spider-Man in What Fictional Character has the best rogues gallery?

Doug Jones

as Chameleon in Spider-Man

Lex Lang

as Doctor Doom in Marvel Animated Universe

Robert Patrick

as Chameleon in Spider-Man Movie Series (1990's)

Nia Long

as Glory Grant in Spider-Man (1990s)

Robert Patrick

as Chameleon in Spider-Man (1990s)

Iona Morris

as Martha Robinson in Spider-Man (1990s)

Tim Russ

as Prowler in Spider-Man (1990s)

Thomas F. Wilson

as Hydro-Man in Spider-Man (1990s)

Ben Kingsley

as Vulture in Peter B. Parker's Universe

John Malkovich

as Vulture in Peter B. Parker's Universe

Anne Hathaway

as Black Cat in Peter B. Parker's Universe

Malcolm McDowell

as Vulture in Peter B. Parker's Universe

David Bradley

as Vulture in Peter B. Parker's Universe