Return to the Spider-Verse

In the four-part episode, "Return to the Spider-Verse" (2016), Madame Web, Iron Fist, and Doctor Strange combine their powers to send Parker and Morales through the multiverse once more to retrieve the fragments before Wolf Spider does. Along the way, they encounter old ally Spider-Man Noir, as well as new Spider-Men like the Victorian era-themed Blood Spider, the cartoon pirate Web Beard, the cowboy Web Slinger, and Spider-Gwen, who became the protector of Morales' universe after he was trapped in Parker's. While in Morales and Gwen's universe, the Web Warriors learn Wolf Spider captured all of the multiverse's Spider heroes so he can use the Siege Perilous to absorb their life forces and empower himself further. Realizing Wolf Spider was poisoning himself in the process, the "prime" Spider-Man allows his life force to be absorbed as well, overloading Wolf Spider and shattering him across the multiverse like the Siege Perilous. With their common enemy defeated, the captured Spiders regain their life forces and return to their respective home universes.