The happiest boy in the playhouse, Pee-Wee Herman couldn't help but feel that reality is getting in the way of him having fun. A darkness will erase all of Puppetland, everything and everyone will be gone and Pee-Wee will turn into a boring depressed adult. Pee-Wee asks Jambi one wish for someone who can help and sends him into the Burton Lands, where he meets the caped crusader, Batman, and their most strangest journey begins.